Identification of the functional phenotype of transgene in kidneys of human tissue inhibitor of metalloproteinase-1 (TIMP-1) transgenic mice
- VernacularTitle:人TIMP-1转基因小鼠肾组织内外源基因功能表型的鉴定
- Author:
Xueguang ZHANG
;
Xiangmei CHEN
;
Yang LU
- Publication Type:Journal Article
- Keywords:
tissue inhibitor of metalloproteinase-1;
transgene;
kidney
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To thoroughly explore the pathophysiological roles of TIMP-1 during the progressive course of renal diseases, the study was aimed at identifying the functional phenotype of endogenous and exogenous genes in kidneys of human TIMP-1 transgenic mice. Methods Renal histological changes between 3-month-old wild type mice (n=8) and 3-month-old transgenic mice (n=8) were analyzed through PAS staining of paraffin sections. The mRNA and protein expressions of h/mTIMP-1, mTIMP-1, TIMP-2, TIMP-3, MMP-9, MMP-2, and COLⅣ?5 mRNA were detected by Northern blot and Western blot. The activities of gelatinases and TIMP-1 were examined by gelatin zymography and reverse zymography, respectively. Results No difference in histological picture in kidneys was found between wild type and transgenic mice. In contrast with wild type mice, it was found that in kidneys of transgenic mice, the mRNA and protein expressions of h/mTIMP-1 and its activity were up-regulated (P0.05). Conclusion The transgene was expressed steadily in kidneys of human TIMP-1 transgenic mice, and it induced the compensation of MMPs/TIMPs.
