Effect of intraperitoneal injection of ?-interferon on the expression of GATA-3 and Th2 cellular factor in murine asthma model
- VernacularTitle:腹腔注射干扰素对小鼠哮喘模型GATA-3及Th2细胞因子表达的影响
- Author:
Yan HUANG
;
Jiquan CHEN
;
Qingyu XIU
- Publication Type:Journal Article
- Keywords:
interferon gamma;
asthma;
GATA-3;
intraperitoneal injections
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of IFN-? in prevention and treatment of bronchial asthma and the mechanism of its effect. Methods BALB/C mice were randomly divided into three groups: group A (control group, n=12); group B (asthma model group, n=12); group C (IFN-? intraperitoneal treatment group, n=12). The asthma model was reproduced in group B and C with ovalbumin (OVA) adsorbed to aluminum hydroxide. PBS (0.25ml) and IFN-? 5?g was respectively injected intraperitoneally in group B and C on days 23 to 30 once daily prior to ovalbumin challenge. Bronchoalveolar lavage fluid (BALF) was collected on day 31 and its cellular composition was analyzed. The levels of IL-4, IL-5 and the IgE in serum were determined. The pathological changes in the lung and the expression of GATA-3 were observed. Results A notable decrease of eosinophils (0.3?0.2) in BALF was found in group C comparing with the group B (21.1?6.7) (P
