Oxidation stress effects of carbon monoxide on rats' hepatic fibrosis induced by carbon tetrachloride
- VernacularTitle:NO在CCl_4致大鼠肝纤维化中的氧化应激作用
- Author:
Jun LI
;
Zhichen CAO
- Publication Type:Journal Article
- Keywords:
liver cirrhosis;
nitric oxide;
oxidative stress;
models, animal;
rats,Wistar
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the oxidation stress effects of nitrogen monoxide (NO) on rats' hepatic fibrosis induced by carbon tetrachloride (CCl_4). Methods Thirty-four male Wistar rats were subcutaneously given CCl_4 to induce hepatic fibrosis model. Six additional rats were chosen to serve as control group. Histological sections of liver of the animals were stained by H & E and Massons staining. NO in serum, malon-dialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue were determined. Results At 7th week, grade 2 or 3 of hepatic fibrosis was reproduced, in which fibrous tissue formed fibrous septa, around the portal areas stretching into hepatic lobules. The structure of the hepatic lobules was intact or disarranged, but there was no signs of cirrhosis. There was moderate fatty change and vacuolar degeneration of heputocytes. The level of serum NO increased significantly (152.8?12.30?mol/L vs. 11.03?1.95?mol/L, P
