Effects of S-adenosylmethionine on hyperhomocysteinemia and the expression of TNF-? in ethanol-induced liver injury in rats
- VernacularTitle:腺苷蛋氨酸对酒精性肝损伤大鼠HHcy和TNF-?的影响
- Author:
Pin ZHANG
;
Yanqing HUANG
;
Xuefen WANG
- Publication Type:Journal Article
- Keywords:
liver diseases, alcoholic;
S-adenosylmethionine;
lipid peroxidation;
cysteine;
tumor necrosis factor
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of S-adenosylmethionine (SAM) on ethanol-induced liver injury in rats. Methods 48 female SD rats were randomly divided into 4 groups as control, model, small and large dose SAM groups. Except control group, all rats were fed high fat-containing diet plus ethanol and fish oil gavage for 8 weeks. SAM was administered by intraperitioneal injection after 4 weeks of exposure to ethanol. Plasma total homocysteine (tHcy), serum aminotransferase activity (ALT), liver malondialdehyde (MDA), glutathione (GSH) contents were assayed, and expression of TNF-? was detected by immunohistochemistry, and liver histology was also examined. Results Compared with control group, model group rats developed marked liver damage, accompanied by an increase of tHcy, ALT, and MDA levels, but a decrease of GSH levels (P0.05). Conclusion SAM prevents alcohol-induced liver injury in rats by reducing liver lipid peroxidation, together with down-regalation of expression of TNF-?. SAM does not affect the plasma total homocysteine levels.
