The role of pulmonary surfactant A in corticosteroids regulating costimulatroy molecules on dendritic cells in murine asthma model
- VernacularTitle:肺表面活性蛋白A在皮质类固醇调节哮喘小鼠树突细胞共刺激分子表达中的作用
- Author:
Binxiao SU
;
Diantao NI
- Publication Type:Journal Article
- Keywords:
glucocorticoids;
pulmonary surfactant-associated protein A;
asthma;
dendritic cells;
costimulatroy molecules
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanisms of corticosteroids regulating costimulatroy molecules on dendritic cells from mouse asthma model, and the role of SP-A in the process. Methods Murine asthma model was established with ovalbumin(OVA) sensitization and challenge, and the model was confirmed by histological analysis of lung tissues. Rabbit antibody against mouse, immunohistochemical ABC method and glucose-DAB-nickel technique for staining and computer image analysis is used to check the expression of pulmonary surfactant A (SP-A) in three groups. FACS was also used to measure the expression of CD-80 on spleen derived dendritic cell from OVA-sensitized and challenged mice. Data was analysised with spss 10.0 software. Results Histological analysis of lung tissues was consistent with the characteristic of murine asthma model. The expression of SP-A in small trachea of asthma group was decreased vs. control group and dexamethasone group(P
