Effect of IGF-1 on the glucose transporter-4 gene expression and protein content in pyaemia rats
- VernacularTitle:IGF-1增加脓毒症大鼠骨骼肌葡萄糖载体及其表达
- Author:
Zhiguang GAO
;
Minfeng LIU
;
Huanlong QIN
- Publication Type:Journal Article
- Keywords:
Pyaemia;
Insulin resistance;
IGF-1;
GLUT-4
- From:
Parenteral & Enteral Nutrition
1997;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influences of IGF-1 on the stress hormone,GLUT-4 and its mRNA expression.Methods: The SD rats with abdominal infection established by cecal ligation and perforation method were randomly divided into three groups: pyaemic group(n=10),parenteral nutrition group(PN group,n=10) and IGF-1 Group(n=10).10 healthy rats was used as the normal group(n=10).On the sixth day,blood was sampled to determine the level of glucose,insulin,glucagon and IGF-1.The expressions of GLUT-4 and its mRNA in muscle were detected by immuno-histochemistry and Real-Time PCR methods.Results: The plasma levels of glucose,insulin and glucagon in the IGF-1 group were significantly lower than those in the pyaemic group and PN group(P
