Selective Effect of Bushen Ningxin Decoction on Mouse Models of Postmenopausal Osteoporosis
- VernacularTitle:补肾宁心方对小鼠绝经后骨质疏松的选择性作用
- Author:
Yudong WANG
- Publication Type:Journal Article
- Keywords:
Bushen Ningxin Decoction;
postmenopausal osteoporosis;
osteoprotegerin;
Th1/Th2;
mRNA expression
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate into the prevention and cure effects of Bushen Ningxin Decoction(BSNXD) on postmenopausal osteoporosis.Methods: Postmenopausal osteoporosis model of female BALB/c mice(8-9 months age) were founded and afforded with the same volume of Chinese traditional medicine(BSNXD),17?-estradiol(E2) and saline(OVX),respectively.The skeleton,blood and uterus of each group were collected after 12 weeks of treatment.The uterus was weighed and analyzed histomorphometrically for differences.The level of IL-4 and IFN-? in serum was detected by ELISA,the bone mineral density(BMD) of excised vertebra(L_(3-4)) and left femurs were measured by DEXA,right femurs were measured for norphometry of bone tissue,and semi-quantitative RT-PCR was performed to compare the level of mRNA level of osteoprotegerin(OPG) from tibia.Results: The level of BMD,area of bone trabecula and OPG mRNA in BSNXD group and E2 group were higher than that of OVX group.The IFN-? level of BSNXD group was improved significantly,and the IL-4 level was reduced comparing to that of OVX group(P
