Expression of recombinant human ?-defensin 2 in dermal multipotent stem cells and its antiseptic activity
- VernacularTitle:重组人?防御素2在真皮多能干细胞中的表达及抗菌活性的测定
- Author:
Nan LI
;
Taoyuan XIAO
;
Yongping SU
;
Hui XU
;
Junping WANG
;
Zhaowen ZONG
;
Xinze RAN
;
Shiwu DONG
;
Zhijun LIU
- Publication Type:Journal Article
- Keywords:
human ?-defensin 2;
recombinant;
dermal multipotent stem cells
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of human ?-defensin 2 (hBD_ 2 ) recombinant adenovirus expression vector in rat dermal multipotent stem cells (dMSCs) and to observe the antiseptic activity of recombinant hBD_ 2 . Methods The expression of hBD_ 2 in dMSCs was examined by RT-PR, fluorescent immunochemistry and Western blotting, and the concentration of recombinant hBD_ 2 in supernate was measured by ELISA. The antiseptic activity of recombinant hBD_ 2 was assessed by K-B disc agar diffusion test. Results hBD_ 2 could be effectively expressed in dMSCs, and the concentration of recombinant hBD_ 2 in supernate was about 743.6 ng/ml . Recombinant hBD_ 2 in supernate showed antiseptic activity. Conclusion Recombinant adenovirus expression vector of hBD_ 2 could be effectively expressed in dMSCs, and the recombinant hBD_ 2 in supernate showed obvious antiseptic effects toward some standard bacteria lines.
