Roles of different n-6/n-3 polyunsaturated fatty acid ratio in gap junctional intercellular communication in breast cancer cell lines
- VernacularTitle:N-6/N-3多不饱和脂肪酸不同比例对乳腺癌细胞缝隙连接细胞通讯的影响
- Author:
Na WEI
;
Mantian MI
- Publication Type:Journal Article
- Keywords:
n-6/n-3 polyunsaturated fatty acid ratio;
breast cancer cell lines;
proliferation activity;
gap junctional intercellular communication
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effects of different n-6/n-3 polyunsaturated fatty acid(PUFA) ratio in gap junctional intercellular communication (GJIC) in breast cancer cell lines. Methods After MCF-7(ER + )and MDA-MB-231(ER - )cells were treated respectively with n-6/n-3 polyunsaturated fatty acid ratio (1-10), Ki-67 expression in nuclear by immunocytochemistry, connexin 26 and 43 expression in cytoplasm and membrane by Western blotting, and GJIC by scrape-loading dye transfer method were performed. Results Immunocytochemistry showed that pure n-6 and 10∶1 n-6/n-3 increased Ki-67 expression (P
