Effects of 3 carbohydrates on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene
- VernacularTitle:三种糖类对新生隐球菌荚膜相关基因CAP10启动活性的影响
- Author:
Zhiyu ZHAO
;
Hai WEN
;
Sunxiao CHEN
- Publication Type:Journal Article
- Keywords:
Cryptococcus neoformans;
capsule;
gene;
promoter;
glucose;
mannose;
galactose
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of glucose,mannose and galactose on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene.Methods: Yeast cells were transfected with plasmid containing a 951 bp length of 5′ upstream flanking sequence of CAP10 coding region and a reporter gene,chloramphenicol acetyl transferase(CAT);the transfectants were treated with different concentrations(10,20,40,60,80 mg/ml) of glucose,mannose and galactose.CAT activity was assessed by ELISA method and CAT activities of different groups were compared.Results: Different concentrations of glucose and mannose had no obvious influence on CAT activity;different concentrations of galactose had obvious influence on CAT activity and the influence was positively dependent with its dose within the experimental concentration range.Conclusion: Glucose and mannose have no obvious effect on the activity of CAP10 promoter;galactose has obvious inductive effect on activity of CAP10 promoter,suggesting that CAP10 gene might be related with galactose metabolism.
