The protective mechanism of Naoxintong on cerebral ischemic injury in rats
- VernacularTitle:脑心通对大鼠脑缺血损伤的保护机制
- Author:
Hongju ZHANG
;
Zhongxin ZHAO
;
Bin XIA
- Publication Type:Journal Article
- Keywords:
striatum;
cerebral ischemia;
GRP78;
GRP94;
Naoxintong
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influence of Naoxintong on expression of glucose-regulated protein 78(GRP78) and GRP94 in the striatum of rats with focal cerebral ischemic injury,so as to investigate their possible protective mechanism on cerebral ischemic injury.Methods:Ninety rats were equally randomized into 3 groups(n=30): sham operated group,ischemic group(Focal transient cerebral ischemia model was established with intraluminal occlusion of left middle cerebral artery) and Naoxintong pre-treated group(Treated with Naoxintong 5 d before ischemic injury).The expression of GRP78,GRP94 in rats striatum was detected by histological method,immunohistochemistry staining and semiquantitative RT-PCR at the different time points(6,12 and 24 h after ischemic treatment).Results:The focal transient cerebral ischemia model was successfully established in rats.Histological results showed that the degree of focal cerebral ischemic injury in Naoxintong pre-treated group was significantly lower than that in ischemic group.Immunohistochemistry staining and RT-PCR results illustrated that the expression of GRP78 and GRP94 in ischemic group was lower than that in sham operated group at each time point after ischemic treatment((P
