In vitro effects of propofol on apoptosis and Bax expression induced by TNF-? in mouse spinal cord neurons
- VernacularTitle:普鲁泊福对TNF-?诱导的小鼠脊髓神经元凋亡及Bax表达的影响
- Author:
Jianhua XIA
- Publication Type:Journal Article
- Keywords:
propofol;
tumour necrosis factor-?;
spinal cord;
neuron;
apoptosis;
Bax
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the in vitro effects of propofol on apoptosis and Bax expression induced by TNF-? in mouse spinal cord neurons.Methods: Spinal cord neurons were isolated from fetal mice and cultured in neurobasal medium with B27 supplement.On the 7th day of culture,neurons were randomly divided into 6 groups:control group,propofol(50 ?mol/L) group,TNF-? group,propofol(25 ?mol/L) with TNF-? group,propofol(50 ?mol/L) with TNF-? group and propofol(100 ?mol/L) with TNF-? group.Propofol with different concentrations was incubated with cultured cells for 30 min,then TNF-? was added with the final concentration of 2 000 U/ml for another 24 h incubation.Apoptosis was detected by PI/Hoechst33342 double staining technique and fluorescence microscopy.Bax expression was determined by immunocytochemical technique.Results: The apoptosis rate and expression of Bax in TNF-? group were both increased compared with those in control group(([21.8?1.1]%) vs [2.8?0.8]%,P
