Protective effect of acanthopanax senticosus saponins on anoxia-damaged motoneurons in rat spinal cord in vitro
- VernacularTitle:刺五加皂苷对体外培养大鼠脊髓运动神经元缺氧损伤的保护作用
- Author:
Jianfeng CHEN
- Publication Type:Journal Article
- Keywords:
acanthopanax senticosus saponins;
apoptosis;
anoxia;
motor neurons;
spinal cord;
cells,cultured
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate whether acanthopanax senticosus saponins(ASS) has a protective effect on anoxia-damaged rat spinal cord motoneurons(SMNs) in vitro and to reveal the possible mechanism of this effects.Methods: SMNs were obtained from the spinal cord of embryonic day 15 rats and were cultured in vitro.The cultured cells were immunohistochemically identified and were subjected to anoxia exposure to establish apoptosis model.In this study,SMNs were divided into the following 4 groups: normal control group;anoxia-induced apoptosis group;ASS pre-treated group,SMNs were treated with ASS(50 ?g/ml) 24 h before anoxia exposure;and glial-cell-line-derived neurotrophic factor(GDNF) pre-treated group,SMNs were treated with GDNF(0.1 ?g/ml) 24 h before anoxia exposure.The morphology of SMNs was observed with phase-contrast microscope and electron microscope;the viability of SMNs was detected with MTT method;and the influence of ASS on the stablility of SMNs membrane was observed through detection of lactate dehydrogenase(LDH) level in the extracellular solution.The influence of ASS on the expression of HIF-1? in anoxia-damaged SMNs was studied by Western blot analysis.Results: The morphological damage of SMNs in ASS and GDNF pre-treated groups was slighter than that in anoxia-induced apoptosis group.The results showed that the viability of SMNs was higher in ASS pre-treated group(0.21?0.028) compared with that in anoxia-induced apoptosis group(0.15?0.012)(P