Protective effects of self-designed Shanghai multi-organ preservation solution on kidney cortex mitochondria function during cold storage
- VernacularTitle:SMO液对低温下肾皮质线粒体功能的保护作用
- Author:
Qiang NIU
- Publication Type:Journal Article
- Keywords:
organ preservation solutions;
cryopreservation;
anoxia;
kidney cortex;
mitochondria
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect of self-designed Shanghai multi-organ preservation solution(SMO) on mitochondria function in canine kidney cortex during cold storage.Methods: Canine kidneys were perfused and stored with cold(0-4℃) SMO,HC-A and UW solution (the later 2 as control) separately.Ultrastructure alteration of mitochondria was observed by electron microscopy.Mitochondria were isolated from cortex homogenization by differential centrifugation and their respiratory function was measured polarographically with Clark oxygen electrode.The four-state curve of mitochondria respiration was drawn and the state Ⅲ and Ⅳ oxygen consumption rate,the respiration control proportion(RCR) and phosphatasel/oxygen ratio(P/O) were calculated.Results: We found that the mitochondria swelling and membrane disruption in HC-A group were more severe than those in SMO and UW group.The rate of oxygen consumption in state Ⅲ,RCR and P/O were obviously decreased in all groups with the prolongation of cold storage time.The rate of oxygen consumption in state Ⅲ in HC-A group was lower compared to that in SMO group during 1-3 d cold storage(P
