Effect of ulinastatin on expression of inflammatory cytokines during perioperative period of surgical correction of adolescent idiopathic scoliosis
- VernacularTitle:乌司他丁对青少年特发性脊柱侧凸患者围手术期炎症细胞因子表达的影响
- Author:
Wei ZHU
;
Xiaoming DENG
;
Wenxian LI
- Publication Type:Journal Article
- Keywords:
ulinastatin;
adolescent idiopathic scoliosis;
tumor necrosis factor;
interleukin
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ulinastatin(UTI) on the changes of IL-6,TNF-?,IL-10 and IL-4 expression during perioperative period of surgical correction of adolescent idiopathic scoliosis(AIS).Methods: Twenty patients with AISⅠ-Ⅱ scheduled to receive surgical correction of spinal deformities were equally randomized into 2 groups-UTI group and control group.Patients in UTI group received intravenous infusion of 10 000 U/kg UTI with 250 ml normal saline just before operation and every 4 h thereafter if necessary;patients in control group received same amount of normal saline.ECG,CVP,SpO_(2)and P_(ET)CO_(2) were continously monitored during operation in both group.Mean arteral pressure(MAP) was maintained at(60?5) mmHg(1 mmHg=0.133 kPa).Venous blood samples were collected immediately before induction of anaesthesia(T_(1)),10 min after induction(T_(2)),1 h after UTI administration(T_(3)),30 min after extubation(T_(4)) and 24 h postoperatively(T_(5)).The plasma levels of IL-6,TNF-?,IL-10 and IL-4 were measured by enzyme-linked immunosorbent assay(ELISA),and their mRNA expression was assayed by real-time quantitative reverse transcription polymerase chain reaction.Results: At T_(3),T_(4) and T_(5),plasma levels of IL-6 and TNF-? and their mRNA copies in control group were significantly higher than that at T_1(P
