Protective effect of pivanampeta on ischemia-reperfusion injury in rat heart
- VernacularTitle:哌芳胺他抗心肌缺血再灌注损伤作用特点及机制
- Author:
Zeling CAO
- Publication Type:Journal Article
- Keywords:
ischemia-reperfusion;
pivanampeta;
cell apoptosis;
heart;
PPAR?;
pioglitazone;
MMP-2
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the feature of pivanampeta (Piv) against ischemia-reperfusion injury with rat heart in vivo and to probe its molecular mechanism.Methods Sprague-Dawley rats were divided into two groups randomly.One was 30 min reperfusion group,which was subdivided into sham,model,vehicle,Piog and Piv with 30 min ischemia followed by 30 min reperfusion to detect some data related to cardiac function and the area of myocardium infarction.Another was 2 h reperfusion group,which was further subdivided into such sections as follows: sham,model,vehicle,Piog,Piv 3,6 and 9 mg?kg~(-1).Besides the sham group,vehicle,Piog,Piv and NS(model) were administered with Piv intravenously 30 min before occlusion.Then hearts were excised,paraffined and cut into 4 ?m.Immunohistochemistry,in situ hybridization,TUNEL and DNA agarose gel electrophoresis were performed to detect the expression of bax,bcl-2,caspase-3,MMP-2 and PPAR? protein and MMP-2,PPAR? mRNA.Results ① Compared with those of model,the ratio of nec/aar after Piv injection decreased 21%(P
