The changes of Na~+,K~+-ATPase activity and ? isoforms in rat neurons suffered from global ischemia
- VernacularTitle:大鼠缺血性全脑损伤Na~+,K~+-ATP酶活性及其?亚基表达的变化
- Author:
Fang GUO
;
Yajuan QI
;
Yongli WANG
- Publication Type:Journal Article
- Keywords:
Na~+,K~+-ATPase;
cerebral ischemia;
? isoform
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of Na~+,K~+-ATPase activity and ? isoforms in rat neurons suffered from global ischemia.Methods The BCAL rat was induced by bilateral carotid artery ligation.The contents of H_2O,Na~+ and K~+ in brain tissue were detected.The method of spectrophotometry was applied to measure the activities of Na~+,K~+-ATPase.With immunohistochemical assay,the expression of the Na~+,K~+-ATPase ?_1,?_2,or ?_3 isoform was detected in neurons of rat hippocampus and cortex following ischemia.Results In the BCAL rats,the contents of H_2O and Na~+ were increased while the content of K~+ and the activity of Na~+,K~+-ATPase were decreased.The ?_1,?_2 or ?_3 isoform of Na~+,K~+-ATPase distributed in a tissue-specific fashion in neurons of hippocampus and cortex,in which ?_1 and ?_3 were abundant and ?_3 was more than ?_1,but ?_2 was much less.In BCAL rat,?_1 and ?_3 isoforms were significantly decreased in hippocampus and cortex neurons.Conclusion These results suggest that the changes of Na~+,K~+-ATPase ?_1 and ?_3 isoforms may be involved in the global cerebral ischemia
