Effect of Smad7 on the expression of TGF-?R,SMADs,and STRAP in the process of malignant transformation of human bronchial epithelial cells
- VernacularTitle:在支气管上皮细胞恶性转化过程中Smad7表达变化对TGF-?R、SMADs及STRAP蛋白的影响
- Author:
Li WANG
- Publication Type:Journal Article
- Keywords:
Smad7;
TGF-?R;
R SMADs;
STRAP;
malignant transformation
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of Smad7 on the expression of TGF-?R,R Smads and STRAP in the process of malignant transformation of immortalized human bronchial epithelial cells BEP2D.Methods Cells were cultured and protein were extracted from BEP2D,BERP35T2,BS7 and RS7 cells,then Western blotting was performed to examine the expression level of TGF-?RⅠ,TGF-?RⅡ,Smad2/3,Smad4 and STRAP.(Results) Stable transfection of Smad7 gene led to decreased expression of TGF-?RⅡ and increased expression of Smad2 and STRAP in both BEP2D and BERP35T2 cells.There were no changes in the expression of Smad3 and Smad4.Conclusion Overexpression of Smad7 leads to decreased expression of TGF-?R Ⅱ and increased expression of STRAP in BEP2D and BERP35T2 cells,which could be one of the mechanisms of the malignant transformation of BEP2D cells.
