Effects of Helicobacter Pylori on mitochondrial cytochrome oxidase subunit Ⅰ in gastric cancer cells
- VernacularTitle:幽门螺杆菌对胃癌细胞线粒体细胞色素氧化酶亚基Ⅰ表达的影响
- Author:
Chunhui LAN
;
Dianchun FANG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
COXⅠ;
gastric cancer cell;
mitochondria
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of concentrated H.pylori culture supernatant(CHCS) on the expressions of COXⅠ mRNA and protein in gastric carcinoma cells,and explore the potential role of H.pylori in the gastric carcinogenesis.Methods After SGC7901 cells were treated by 6 ?l/ml CHCS for 4 h,8 h,12 h respectively,the expression of COXⅠmRNA and protein was detected by RT-PCR and Western blotting.Results The expression of COXⅠmRNA decreased gradually after exposure to CHCS for 4 h.Especially after 8 h,the expression decreased significantly.When treated with CHCS for 12 h,the expression continued to decrease(P
