Overexpression of glucocorticoid receptor beta affects regulatory effect of glucocorticoids on proliferation and differentiation of human osteosarcoma cells
- VernacularTitle:糖皮质激素受体?的稳定过度表达对激素调节人骨肉瘤细胞增殖分化作用的影响
- Author:
Yun ZHOU
- Publication Type:Journal Article
- Keywords:
receptors,glucocorticoid;
osteosarcoma;
proliferation;
differentiation
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of glucocorticoid receptor beta overexpression on the regulatory effect of glucocorticoid on proliferation and differentiation of human osteosarcoma cell and to investigate the biological significance of glucocorticoid receptor beta.Methods: Glucocorticoid receptor beta was stably transfected into human osteosarcoma cells and the stable transfectants were treated with different concentrations of dexamethasone(10~(-10)-10~(-6) mol/L).Then cell proliferation was determined by MTT method and viable cell counting;alkaline phosphatase was detected by standard hydroxybenzene colorimetry.Results and Conclusion: In stable transfectants overexpressing glucocorticoid receptor beta,glucocorticoid-induced cell proliferation repression and cell differentiation are inhibited,indicating that glucocorticoid receptor beta may be an endogenous inhibitor of glucocorticoid receptor alpha.
