Preparation of a novel ketoconazole spraying membrane and its effect on eczema and tinea corporis in guinea pigs
- VernacularTitle:新型酮康唑喷膜的制备及其对湿疹和体癣豚鼠模型的疗效
- Author:
Xuemei YI
- Publication Type:Journal Article
- Keywords:
ketoconazole;
desamethasone;
spraying membrane;
eczema;
tinea corporis
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a novel slow-releasing ketoconazole spraying membrane and to observe its effect on eczema and tinea corporis in guinea pigs.Methods: A novel ketoconazole spraying membrane was prepared with chitosan chlorhydrate as the membrane material.The contents of ketoconazole and desamethasone in the spraying membrane were assayed by HPLC.The separation was performed on Waters Symttry C_(18) column(250 mm?4.6 mm,5 ?m),with the mobile phase consisting methanol-potassium dihydrogen phosphate buffer(4∶1),at a flow rate of 0.6 ml/min.The dectection was carried out with a UV detector at 239 nm,at(22?2)℃.The quality criteria for the novel ketoconazole spraying membrane was established.The curative effect of the ketoconazole spraying membrane on eczema and tinea corporis was observed in guinea pigs and was compared with those of spraying membrane matrix,desamethasone antipruritics cream and compound ketoconazole cream.Results: The calibration curves of ketoconazole and desamethasone were linear between 0.2-2.0 mg?ml~(-1)and 0.02-0.20 mg?ml~(-1),respectively;the recoveries for ketoconazole and desamethasone were 98.41% and 97.84%,respectively.The intra-day and extra-day RSDs for ketoconazole were(1.17%) and 1.26%,respectively,and for desamethasone were 1.57% and 1.40%,respectively.Stimulation test showed that the spraying membrane had no irritative effect on the skin of guinea pig.The novel ketoconazole spraying membrane had similar effect on eczema and tinea corporis as those of desamethasone antipruritics cream and compound ketoconazole cream.Conclusion: This novel ketoconazole spraying membrane is easy to prepare and has obvious therapeutic effects on eczema and tinea corporis in guinea pigs,making it favourable for containing combined agents.
