Activity of PI3′K/Akt/FKHRL1 signaling pathway induced by doxorubin confer chemoresistance in gastric cancer cell.
- VernacularTitle:阿霉素诱导磷脂酰肌醇3′-激酶丝氨酸/苏氨酸蛋白激酶FKHRL1通路对胃癌细胞化疗耐药性的影响
- Author:
Jing CHEN
;
Honggang YU
;
Jieping YU
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Doxorubin;
Phosphatidylinositol-3kinase Serinethreonine kinase;
Chemoresistance
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between phosphatidylinositol-3kinase(PI3′K)/serine/threonine kinase(Akt)/Forkhead like 1(FKHRL1)signaling pathway and chemoresistance in human gastric cancer cell line SGC7901.Methods From Jan.2004 to sep.2005,cells were exposed to doxorubicin with or without Wortmannin(a special inhibitor of PI3′K/Akt pathway)in Department of Gastroenterology the people's Hospital of when University.Cytotoxicity was assessed by determining cell survival with MTT.By Western blot analysis,the phosphorylation levels of FKHRL1 was evaluated in SGC-7901 cell.Results It was found that doxorubicin caused reduction of cell viability of SGC-7901 and induced phosphorylation of FKHRL1 in a time-dependent manner.Wortmannin enhanced the cell inhibitory efficiency of doxorubicin.Phosphorylation levels of FKHRL1 was significantly induced by Doxorubin in a time-dependent manner and was blocked by wortmannin.Conclusion Doxorubin may activate PI3′K/Akt signaling pathway and then induce phosphorylation of FKHRL1 in a time-dependent manner,which affects the chemoresistance of gastric cancer cell .However,Wortmannin enhances the chemotherapy sensitivity by suppressing this pathway.
