Preparation of replication-deficient recombinant adenovirus coding ARP2 gene.
- VernacularTitle:人血管生成素相关蛋白2重组腺病毒制备
- Author:
Shu MENG
;
Jiang CAO
;
Shengdong HUANG
- Publication Type:Journal Article
- Keywords:
ARP2;
Recombinant adenovirus
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the replication-deficient recombinant adenovirus coding ARP2 gene before the studies of gene transfection to ischemic myocardium.Methods From Dec.2004 to Oct.2005,in the Department of Cardiology,Changhai Hospital of the Second Military Medical Univesity,the ARP2-pAxCAwt was constructed by inserting the cDNA of interest into the SwaI site of pAxCAwt.Results The right direction of the insert was confirmed by restriction.Conclusion The direction of the insert must be confirmed by restriction analysis,and the DNA package protein is much helpful during the transfection of the recombinant cosmid to E.coli.
