Study on mechanism of human bone mesenchymal stem cell in treating patients with aplastic anemia.
- VernacularTitle:人骨髓间充质干细胞治疗再生障碍性贫血的机制研究
- Author:
Yi DONG
;
Ruixiang XIA
;
Qingshu ZENG
- Publication Type:Journal Article
- Keywords:
Anemia;
Aplastic;
Mesenchymal stem cells;
T cells
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore mechanism of human bone mesenchymal stem cell(MSC)in treating patients with aplastic anemia(AA)in vitro.Methods MSCs were separated with Percoll(1.073 g/mL)and cultured in low glucose DMEM.T cells were harvested by using nylon column.MSCs of various concentrations were added to PHA induction T cell proliferation cultures with AA patients.The proliferation of T cell was measured by MTT method. The CD25(IL-2R)expression rates of CD~+_3 T cells was analyzed by flow cytometry .Results MSCs were planted in 96-well plates(2?10~4/well for group A,1?10~4/well for group B)and cocultured with T cell isolated from peripheral blood of AA patients. Peripheral blood T cell noncocultured with MSC acted as the control group.MSCs significantly inhibit PHA-induced T cell proliferation and the CD25 expression of CD~+_3 T cells in patients with AA(P
