A study of modulation of white blood cell immune function by erythrocyte with a new system of immunological experimental system
- VernacularTitle:红细胞调控白细胞免疫功能新的自然实验研究体系
- Author:
Feng GUO
;
Lezhi ZHANG
;
Zhiyang CAI
- Publication Type:Journal Article
- Keywords:
allergy and immunology;
erythrocytes;
leukocytes;
interleukin-8;
interleukin-12;
antigens, CD4;
receptors, complement 3b
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate of red blood cell as giving instruction in whole white blood cell immunological activity by new nature experimental system of hemaimmune reaction rood map. Methods Plasma 0.3ml were added to whole blood cells (including: red blood cell and white blood cells) or white blood cells 0.2ml, and incubated for 1h at 37℃. The content of IL-8 and IL-12 was determined by enzyme linked immunadsorbent assay (ELISA) method. The expression level of CD4, CD8, CD35 and CXCR4 on white blood cells was determined by method of Flow Cytometry. Results The content of IL-8 (5.96?4.26) and IL-12 (9.84?2.23) in whole blood and plasma nature group was significantly lower than that (13.59?3.69?B?pg~ -1 ?ml~ -1 ) and (15.09?9.86?B?pg~ -1 ?ml~ -1 ) in white blood cell and plasma isolation group (P
