The effects of erythrocyte CD35 on the immuno-response of lymphocyte activated by antigen
- VernacularTitle:红细胞CD35在抗原激活淋巴细胞免疫反应中的作用
- Author:
Zhanshan ZHA
;
Baohua QIAN
;
Feng GUO
- Publication Type:Journal Article
- Keywords:
allergy and immunology;
erythrocytes;
receptors, complement 3b;
lymphocytes
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of CD35 on erythrocytes played in antigen activating immunological reaction of lymphocytes. Methods Suspensions of lymphocytes(1?10~6/ml)and erythrocytes (1?10~8/ml) were respectively separated from anticoaguted whole blood of healthy adults with the lymphocyte separation medium. Using inactivated ascites carcinoma cell S180(1?10~6/ml) as activation antigen and autologous plasma as reactive medium, the role of erythrocytes in regulating the anti-neoplasm immunological reaction of lymphocytes was appraised. The expression of CD25 on lymphocytes was detected and compared using flow cytometry assay with CD35 on erythrocytes blocked by monoclonal antibody or complements in plasma were destroyed by EDTA. Results The expression of CD25 on lymphocytes (18.22?4.27%) was significantly decreased when CD35 on erythrocytes was blocked (P
