Study on the effect of the combination of thalidomide and As_2O_3 on VEGF expression in ANLL
- VernacularTitle:沙利度胺联合三氧化二砷对急性非淋巴细胞白血病VEGF表达的影响
- Author:
Huan WANG
;
Yingchun LI
;
Wei YANG
- Publication Type:Journal Article
- Keywords:
VEGF;
Thalidomide;
As_2O_3
- From:
Chinese Journal of Practical Internal Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of thalidomide and As_2O_3 to the secretion of VEGF of the cell of ANLL and study if there is coorperation in thalidomide and As_2O_3.Methods From October 2001 to February 2003,23 patients with ANLL were analyzed.Heparinized marrow was taken,then it was putt into Ficoll-isopaque solution to be purified for MNC,which was cultured in the RPMI 1 640 medium,placed in 5% CO_2 saturation and 37℃ to proliferation.Choosing the cells in logarithm growth phase,adding cultural solution to abstaining the cellular density of 2?10~9/L and then putting it into 24-well plates.There are 4 groups :contrast group,thalidomide(300 ?g?mL~ -1 )group,As_2O_3(25 ?mol/L)group,and thalidomide(300 ?g?mL~ -1 )andAs_2O_3(25 ?mol/L)group,cultured for 72 hours.Examine the concentration of VEGF in upper liquid with ELISA method.Results The concentration of VEGF in upper liquid of 4 groups were respectively(1 582.67?281.51)ng/L、(1 206.29?264.87)ng/L、(941.72?236.34)ng/L、(639.12?211.98)ng/L.There were statisticd difference(P
