pSilence APE1 inhibits migration of endothelial cell induced by osteosarcoma cell
- VernacularTitle:pSilence APE1对骨肉瘤诱导血管内皮细胞迁徙抑制作用的实验研究
- Author:
Dong WANG
;
Zhaoyang ZHONG
;
Zengpeng LI
;
Yi QING
;
Qinhon ZHANG
- Publication Type:Journal Article
- Keywords:
bone tumor;
APE1;
angiogenesis
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the inhibitory effect of APE1 siRNA expression vector pSilence APE1, and the possible synergetic role of pSilence APE1 and endostatin on endothelial cell migration induced by osteosarcoma cell 9901 and HOS . Methods The osteosarcoma cells and endothelial cells were co-cultured with transwell model, and the cell number through the inner membrane was counted to evaluate the inhibitory effect of endothelial cell migration by pSilence APE1 and its combination with endostatin. Results Both low dose (350 ng/ml) and high dose (700 ng/ml) of endostatin showed significant inhibition to endothelial cell migration induced by osteosarcoma cell, while the high dose showed much stronger inhibition than the low dose (P
