Influence of perdipine on the proliferation and PYK_2 expression in cultured adult rat cardiac fibroblast stimulated by angiotensin Ⅱ
- VernacularTitle:盐酸尼卡地平对血管紧张素Ⅱ诱导成年大鼠心脏成纤维细胞增殖和PYK_2表达的影响
- Author:
Jia HAO
;
Wei LU
;
Yingbi XIAO
- Publication Type:Journal Article
- Keywords:
perdipine;
cardiac fibroblast;
angiotensin Ⅱ;
focal adhesion kinase
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of perdipine on the proliferation and proline-rich tyrosine kinase 2 (PYK_ 2 ) expression in cultured adult rat cardiac fibroblast stimulated by angiotensin Ⅱ, and to explore the mechanism of perdipine on cardiac fibrosis. Methods Adult rat cardiac fibroblasts (CFs) were treated with angiotensin Ⅱ(AngⅡ) to establish fibrosis model. The effects of perdipine on proliferation of CFs were analysed by MTT colorimetric assay, and fibronectin was tested by immunohistochemistry method. PYK_ 2 mRNA and protein level were examined by RT-PCR and Western blot analysis respectively. Results Perdipine inhibited CFs proliferation and fibronectin synthesis stimulated by angiotensin Ⅱ in a dose dependent, and the levels of PYK_2 mRNA and protein were decreased significantly. Conclusion Perdipine can inhibit cardiac fibrosis stimulated by angiotensin Ⅱ by suppressing CFs proliferation and fibronectin synthesis. PYK_2 is involved in the effect of perdipine.
