The phenotype changes of SGC7901 gastric cancer cell-dendritic cell fusion vaccine
- VernacularTitle:SGC7901-DC融合细胞疫苗表型变化特点的研究
- Author:
Kun ZHANG
;
Peiwu YU
;
Pengfen GAO
;
Yu RAO
- Publication Type:Journal Article
- Keywords:
SGC7901;
dendritic cell;
fusion vaccine;
phenotype
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain SGC7901 gastric cancer cell-dendritic cell fusion vaccines by cell fusion technology. The phenotype changes of the fusion cells were detected to provide experimental data for the following research work. Methods The SGC7901 gastric cancer cells and dendritic cells were induced to be fused by PEG and the pure fusion cells were obtained by selective culture with the HAT/HT culture system. The phenotype changes of the fusion cells were detected by flow cytometer. Results The fusion cells had special morphologic characteristic and the phenotypes of the fusion cells were remarkably higher than the parental dendritic cells. Conclusion The SGC7901 gastric cancer cell-dendritic cell fusion cells kept special morphologic characteristic and their phenotypes were significantly higher than the parental dendritic cells, which may be the cellular base for their stronger biological effects.
