Construction of Eukaryotic Expression Vector Containing Human Vascular Endothelial Growth Factor 165 Gene
- VernacularTitle:人血管内皮细胞生长因子165基因真核表达载体构建
- Author:
Bo WU
;
Bing CHEN
;
Qunying XIONG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
Clone;
Gene expression
- From:
Journal of Medical Research
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone human vascular endothelial growth factor 165(hVEGF_ 165 ),construct its eukaryotic expression and to study the expression of hVEGF_ 165 . Methods Human vascular endothelial growth factor cDNA was amplified by PCR method from the HL60 cells and cloned to expression vector pcDNA_3,constructed pCD-hVGEF_ 165 recombinant plasmid, then transformed to E.coli BL21(DE3)cell.Results The cloned cDNA was confirmed to be VGEF_ 165 cDNA. It was observed that the expression of human VEGF gene was detected distinctly 72h after transferring.Conclusions We successfully cloned and expressed hGEF_ 165 gene, which provided the further foundation of the model of VEGF transgenic animal and makes a basis for the further study in retinal neovascularization.
