RP-HPLC determination of ferulate acid in the compound angelica scinensis pian
- VernacularTitle:反相高效液相色谱法测定复方当归片剂中阿魏酸的含量
- Author:
Ji YANG
;
Jinchun SONG
;
Yuguang WANG
;
Hong LIU
- Publication Type:Journal Article
- Keywords:
Chromatography,high pressure liquid;
Pharmacology,clinical;
Fufangdanggui;
Ferulate acid
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine ferulate acid in the compound angelica scinensis particle by RP-HPLC method.Methods The liquid chromatography was carried out using Zorbax C_ 18 (5?m,4.6mm?250mm),with detection wavelength at 320nm.The flow rate of mobile phase[5% acetic acid-methanol(75∶25)] was 1ml/min.Results The calibration curve was linear in the range of 0.0141～0.450mg/ml(r=0.9997,n=5).The average recoveries was 94.87%(RSD=0.5%).Conclusion The method establishied in this paper can be adopt for the compound angelica scinensis particle.This method is simple,accurate,reliable and reproducible.
