Effect of medroxyprogesterone acetate on proliferation and apoptosis of human colorectal cancer cells
- VernacularTitle:醋酸甲羟孕酮对人结肠癌细胞增殖和凋亡的影响
- Author:
Weijun WANG
- Publication Type:Journal Article
- Keywords:
medroxyprogesterone acetate;
colonic neoplasms;
cell proliferation;
apoptosis
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of medroxyprogesterone acetate(MPA) on the proliferation and apoptosis of human colorectal cancer cells.Methods: Human colorectal cancer cell line LS174T was treated with different concentrations of MPA(12.5,25,50 and 100 ?mol/L) for 120 h.The inhibitory effect of MPA was measured with MTT method;the cell cycle,cell apoptosis,and cell surface Fas expression were analyzed with flow cytometry.Results: MPA had significant inhibitory effect on the growth of LS174T human colon cancer cells.Cell growth was found to be arrested in G_(1) phase and cells in S phase and G_(2)/M phase were decreased.The natural apoptosis rate and the apoptosis rates at MPA 12.5 and 25 ?mol/L were 4.8%,15.1%and 25.6%,respectively.Cell surface Fas expression in cancer cells increased after treated with MPA.Conclusion: MPA has significant inhibitory effect on the growth of human colon cancer cells,which may be related to Fas expression upregulation on cancer cell surface and G_(1) phase arrest.
