Primary active components in sepia: a qualitative and quantitative analysis
- VernacularTitle:乌贼墨中主要活性成分的定性定量分析
- Author:
Yunyun JIANG
- Publication Type:Journal Article
- Keywords:
sepia;
amino acids;
iron;
chromatography,high pressure liquid;
spetrophotometry,atomic
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for qualitative and quantitative analysis of the major active components in sepia.Methods: Melanin was identified by dissoving it in a basic solution to see whether there was segregation of a black substance.Glycine,asparagines,glutamic acid and alanine in sepia were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer.Results: Melanin in sepia was dissolved in 2 mol/L NaOH solution and black substance was obtained in 2 mol/L HCl solution.The recoveries of the above 4 amino acids and iron were within the range of 95%-105% with RSD
