Effects of Fufangdanshen on TNF-? stimulated vascular smooth muscle cells using proteomics
- VernacularTitle:复方丹参方对TNF-?刺激血管平滑肌细胞影响的蛋白质组学研究
- Author:
Zengchun MA
;
Yue GAO
;
Hongling TAN
;
Chengrong XIAO
;
Jie WANG
;
Xuemin ZHANG
;
Shengqi WANG
- Publication Type:Journal Article
- Keywords:
fufangdanshen;
TNF-?;
cell proliferation;
atherosclerosis
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Fufangdanshen on TNF-? stimulated vascular smooth muscle cells(VSMC) and discover potential molecular mechanism to cure atherosclerosis at molecular level.Methods TNF-? stimulated VSMC was used as dysfunction cell model;VSMC proliferation was detected with MTT and FACS;proteomic protocol involving 2-DE,image analysis and spectrometry detection were used to detect regulated protein by Fufangdanshen.Results Fufangdanshen inhibited expression of 16 proteins but promoted expression of 24 proteins in TNF-? stimulated VSMC.The expression of CaMKK and N-ras were decreased by Fufangdanshen,which farther decreased matrix metalloproteinase production;while cell cycle related protein p21,p53 were increased by Fufangdanshen in TNF-? treated VSMC.Conclusion The inhibition of proliferation and migration of VSMC through decreased matrix metalloproteinase production and increased cell cycle related protein p21,p53 might be the mechanism of Fufangdanshen to cure atherosclerosis.
