Effect of Losartan and CGRP on the proliferation of vascular smooth muscle cell induced by angiotensin Ⅱ
- VernacularTitle:Losartan和CGRP对血管紧张素Ⅱ诱导血管平滑肌细胞增殖作用的影响
- Author:
Xiaoyi ZHANG
;
Yuhuan LIU
;
Duanfang LIAO
;
Xuping QIN
- Publication Type:Journal Article
- Keywords:
losartan;
CGRP;
vascular smooth muscle cell;
proliferation
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To compare the effects of the non-peptide angiotensin Ⅱ receptor type Ⅰ antagonist,Losartan,and the active vascular peptide,calcitonin gene-related peptide(CGRP),on the proliferation of vascular smooth muscle cells induced by angiotensin Ⅱ,and to explore the mechanism of depressor effect of Losartan and CGRP in vivo.Methods MTT,Thymidine incorporation and flow cytometry,were used to determine the ability of proliferation of VSMC induced by angiotensin Ⅱ in the presence or absence of Losartan or CGRP,Western blotting was used to determine the activity of ERK1/2.Results Losartan or CGRP inhibited the viability,DNA synthesis,cell proliferation index,and the activity of ERK1/2 in a dose-dependent manner.Conclusion Losartan or CGRP significantly inhibits the proliferation of VSMC induced by angiotensin Ⅱ;the inhibitory effect of CGRP is stronger than that of Losartan.The signaling path way is involved in ERK1/2.
