Regulation of ERK MAPK in oridonin-enhanced phagocytosis of apoptotic bodies in human macrophage-like U937 cells
- VernacularTitle:冬凌草甲素增强U937细胞吞噬凋亡小体过程中ERK途径的调节作用
- Author:
Yanqiu LIU
- Publication Type:Journal Article
- Keywords:
oridonin;
U937 cell;
phagocytosis;
ERK
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the mechanisms of oridonin-enhanced phagocytosis of apoptotic bodies by macrophage-like U937 cells.Methods Photomicroscopical observation,PKC activity assay,acridine orange staining,Hoechst 33258 staining,and Western blot analysis were used.Results Oridonin-augmented phagocytosis was suppressed by either protein tyrosine kinase(PTK) inhibitor genistein or protein kinase C(PKC) inhibitor stauroporine.Exposure of U937 cells to 2.7 ?mol?L~(-1) oridonin caused an increase in PKC activity time dependently.In addition,ERK inhibitor PD98059 blocked phagocytic stimulation as well as the increased expression of phsophorylated ERK in response to oridonin.Conclusion Oridonin enhanced macrophage-like U937 cells to ingest apoptotic bodies by upregulation of PTK activity and thus PKC activation,resulting in phosphorylation of the downstream mediator,ERK.
