Increased expression of podocyte molecules might cause proteinuria in adriamycin-induced nephrotic rats
- VernacularTitle:足细胞分子高表达致阿霉素肾病大鼠发生蛋白尿
- Author:
Yan XING
;
Jie DING
;
Qingfeng FAN
;
Na GUAN
- Publication Type:Journal Article
- Keywords:
Proteinuria;
Nephrotic syndrome;
Actinin;
Podocyte molecule
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To dynamically observe the expression of slit diaphragm complex molecules, including nephrin, podocin, CD2AP, and cytoskeleton protein a-actinin-4, in adriamycin-induced nephrotic (ADN) rats, and to further explore the molecular behavior of podocyte proteins during the occurrence and development of proteinuria and their possible mechanisms. Methods Adriamycin nephropathy was induced by a single tail intravenous injection of adriamycin. Renal tissue samples were collected at day 3, 7, 14, and 28, respectively. The distribution, mRNA expression and protein expression of nephrin, podocin, CD2AP and a-actinin-4 were examined by indirect immunofluorescence, real-time PCR and Western blotting, respectively. Results (1) After the adriamycin injection, a significant increment of the 24-hour urinary protein was observed at day 14 and persisted up to day 28 (P
