Expression of ANGPTL3 mRNA in kidney of adriamycin-induced nephrotic rats
- VernacularTitle:阿霉素肾病大鼠肾组织中血管生成素样蛋白3基因表达
- Author:
Jianwen WU
;
Hong XU
;
Xiaoqing ZHAO
;
Xiaoyan ZHOU
;
Ying WU
- Publication Type:Journal Article
- Keywords:
Nephrotic syndrome;
Angiopoietins;
Animal, model;
Gene,microdissection
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of angiopoietin-like protein 3(ANGPTL3) mRNA successively in kidney of adriamycin (ADR)-induced nephrotic rats during the development of proteinuria, and to disclose the possible assosiation of ANGPTL3 with proteinuria. Methods Adriamycininduced nephrotic rat models were established by a single injection of adriamycin via the tail vein. Glomeruli and cortex tubuli of rats were dissected by laser microdissection. Real time quantitative RT-PCR was used to study the expression of ANGPTL3mRNA in kidney of ADR rate at 7, 14, 21 and 28 days successively following adriamycin injection. Results (1) In ADR rats, urinary protein and the level of triglyceride and cholesterol in serum increased significantly at day 14 (P
