Study of K~(+) channels at the apical membrane of mouse renal proximal tubule cell with patch-clamp
- VernacularTitle:小鼠肾近曲小管上皮细胞顶侧膜钾通道的膜片钳研究
- Author:
Yali LIU
;
Wei HUANG
;
Enquan GAO
;
Benlan YE
- Publication Type:Journal Article
- Keywords:
renal proximal tubule;
potassium channel;
patch clamp
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the property of the K~(+) channels at the apical membrane of mouse renal proximal tubule cells.Methods Single-channel recording of the K~(+) channel currents in apical membrane of freshly isolated renal proximal tubule cells was performed using the cell-attached mode of the patch-clamp technique.Results This kind of potassium channel appeared to be an inward rectifier,and its limiting inward slope conductance was(11.4?0.6) pS.The channel activity increased with hyperpolarization.Conclusion The potassium channel with spontaneous current was observed in most of the cell-attached patches,which is thought to be involved in volume regulation,potassium recycling and stabilization of the apical potential.
