Dendritic cells pulsed with whole tumor lysates provide protection against acute leukemia in syngeneic bone marrow-transplanted mice
- VernacularTitle:肿瘤抗原冲击致敏的树突状细胞在骨髓移植小鼠中抗白血病的免疫反应
- Author:
Yirong JIANG
;
Chunsheng LIU
;
Xueliang CHEN
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Bone marrow transplantation;
Leukemia
- From:
Chinese Journal of Practical Internal Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate specific anti-leukemia immune response induced by tumor lysates pulsed dendritic cells(TP-DC)in syngeneic bone marrow-transplanted mice.Methods From June 2002 to June 2003,the bone marrow cells were induced by using granulocyte-macrophage colony stimulating factor(mGM-CSF)and interleukin-4(mIL-4).DC was pulsed with L7212 tumor lysates at the immature stage.TP-DC was harvested on 7th day.615 mice immunized thrice with L7212 TP-DC starting at day 7 following BMT.Additional control groups were set up.The survival was observed.Results Immunization using TP-DC could induce L7212 cells-specific CTL responses that were statistically significant compared with control groups(F=391.77,P
