Apoptosis of primary spinal cord neurons of rat embryo before and following injury in vitro
- VernacularTitle:体外培养大鼠胚胎脊髓神经元损伤前后的凋亡变化
- Author:
Haiping QUE
;
Bingyao CHEN
;
Shaojun LIU
- Publication Type:Journal Article
- Keywords:
spinal cord injuries;
neurons;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis rate of neurons in vitro among the primary culture cells isolated from rat spinal cords before and after injury. Methods The spinal cord neurons of Wistar rats at 14-day gestation were isolated and cultured. The neuronal processes were then injured by cutting. At different time points after injury, TUNEL method was employed to detect the apoptotic neurons. Results Before injury, there was almost no apoptotic neuron. However, a large amount of apoptotic neurons were observed after the injury. The highest incidence of apoptosis appeared on the first day, and then it gradually reduced in the following days, but increased in amount again on the seventh day. Conclusion The results of the present experiment reveal the regularity of apoptosis of neurons before and after injury, and it provides a platform for further research regarding therapeutic intervention in the treatment of spinal cord injury.
