Effect of recombinant rat augmenter of liver regeneration on proliferation and apoptosis of renal tubular cells
- VernacularTitle:重组大鼠肝再生增强因子对肾小管上皮细胞增殖及凋亡的影响
- Author:
Xiaohui LIAO
;
Ling ZHANG
;
Qi LIU
- Publication Type:Journal Article
- Keywords:
Liver regeneration;
Renal tubules;
Epithelial cells;
Gentamicin;
Apoptosis;
Proliferation
- From:
Chinese Journal of Nephrology
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of recombinant rat augmenter of liver regeneration (rrALR) on proliferation and apoptosis of renal tubular cells in vitro. Methods The cultured human renal tubular cell line (HK2 cells) was divided into several groups with various concentration of gentamicin(GM) or/and rrALR. The proliferation of HK2 cell was evaluated by 3H-TdR incorporation. The apoptosis of HK2 cells was assessed by AO/EB staining and flow cytometer using Annexin V-FITC and propidium iodide (PI) staining. Results (1 ) rrALR promoted HK2 cell proliferation in a time-dependent and dose-dependent manner. The proliferative effect was significant with the concentration from 25 ng/ml to 50 ug/ml (P
