Estabishment and characterization of mouse hybridoma cell lines secreting monoclonal antibody against annexin B1
- VernacularTitle:Annexin B1单克隆抗体杂交瘤细胞株的建立和特性鉴定
- Author:
Yuanjian GAO
- Publication Type:Journal Article
- Keywords:
Annexin B1;
hybridomas;
monoclonal antibody
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare monoclonal antibody against Annexin B for further studying the physiological function of Annexin B1.Methods: Annexin B1 was expressed and purified in E.coli and its antigenicity was confirmed.The product was used to immunize BALB/c mice by intrasplenic embedding.Spleen cells of BALB/c mice were obtained and fused with myeloma cell line SP2/0 in a HAT medium containing 50% PEG 4000 and were then subjected to incubation in 37℃,5% CO_(2)incubator for several days.ELISA was used to assay the type and titer of the McAbs in the supernatant.Results: A hybridoma cell line 2B10H5,which can steadily secrete specific McAbs,was obtained.Conclusion: The McAbs of Annexin B1 have been successfully prepared,which pave a way for further investigation of Annexin B1.
