Lovastatin in regulation of mesangial cell proliferation and activity of TGF-?1 promoter in rats
- VernacularTitle:洛伐他汀对大鼠系膜细胞增殖及TGF-?1基因启动子活性的调控
- Author:
Hai YU
- Publication Type:Journal Article
- Keywords:
lovastatin;
tranforming growth factor ?;
promoter;
kidney;
mesangial cell;
cell proliferation
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of lovastatin on the proliferation of rat mesangial cell(RMC) and the activity of TGF-?1 gene promoter.Methods:(1)Effect of lovastatin(10~(-7),10~(-6),10~(-5) and 10~(-4) mol/L) on proliferation of RMC was analyzed by MTT.(2)Two plasmids(phTGF2.14 and phTGF1.12) containing various lengths of 5′flank sequences of human TGF-?1 gene and CAT as reporter gene were constructed and transfected into RMC by FuGENE transfection reagent.The effects of lovastatin(10~(-7),10~(-5)mol/L)on transcription of the 2 plasmids were studied by CAT-ELISA.Results:(1)Different concentrations of lovastatin had obvious inhibitive effects on the proliferation of RMC compared with control group(P
