Role of pioglitazone in resolution of monosodium urate monohydrate crystal induced acute gouty arthritis in rats
- VernacularTitle:吡格列酮对大鼠急性痛风性关节炎的防治作用
- Author:
Huogao HUANG
- Publication Type:Journal Article
- Keywords:
peroxisome proliferator-activated receptor gamma;
pioglitazone;
arthritis,gouty
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the feasibility and mechanism of pioglitazone,an agonist of peroxisome proliferator-activated receptor ?(PPAR?) in resolution of monosodium urate monohydrate(MSU) crystal-induced arthritis.Methods: A rat model of acute gouty arthritis was induced with the injection of 1mg(20 mg/ml?50 ?l) MSU into rat ankle joint cavity.Dynamic mRNA expressions of PPAR? in synovium at 0 h,4 h,8 h,24 h and 48 h after MSU injection were determined by reverse transcriptase-polymerase chain reaction(RT-PCR).The effects of pioglitazone(4 mg?kg~(-1)?d~(-1) and 20 mg?kg~(-1)?d~(-1)) on arthritis were investigated through assessing clinical features and histopathologic changes in rats at 2 h,4 h,6 h,8 h,10 h,12 h,24 h and 48 h after arthritis induction.Results: The mRNA expression of PPAR? in synovium of rats with arthritis was significantly increased in a time-dependent manner and peaked at 48 h after MSU injection.The anti-arthritis effects of pioglitazone were not significant during 24 h after induction,and high dose other than low dose of pioglitazone significantly ameliorated the inflammation,swelling,disability index and histopathologic changes at 48 h after MSU injection in rats.Conclusion: Pioglitazone has obvious anti-inflammatory properties in gouty arthritis.Elevated expression of PPAR? in synovium after arthritis induction may contribute to effects of PPAR? agonist and resolution of acute attacks of gout.
