Study on the apoptosis of cervical cancer HeLa cells transfectead by comol.L-1 bination of telomol.L-1erase anti-hTR and anti-hTERT
- VernacularTitle:端粒酶反义hTR和反义hTERT合用对宫颈癌HeLa细胞凋亡的影响
- Author:
Zhongdong CHEN
;
Lixiu LEI
- Publication Type:Journal Article
- Keywords:
cervical cancer;
telomerase;
antisense oligonucleotides;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the apoptosis of cervical cancer HeLa cells cultured in vitro transfectead by combining telomerase anti-hTR and anti-hTERT. Methods The activity of telomerase was tested by polymerase chain reaction-telomeric repeat amplification protocolenzyme-linked immunosorbent assay (PCR- TRAP- ELISA). Cell morphologies were observed by fluorescence microscope stained with acridine orange. Apoptosis and cell cycle were examined by Flow Cytometer method (FCM). Results The telomerase activity of HeLa cells transfected by antisense oligonucleotides were significantly decreased compared with those of nontreated control,sense oligonucleotides and oligofectamine TM(P
