EICOSAPENTAENOIC ACID ALTERS INTERLEUKIN-2 RECEPTOR DISTRIBUTION IN DETERGENT-INSOLUBLE MEMBRANE DOMAIN
- VernacularTitle:二十碳五烯酸影响T细胞膜脂肪微区域中IL-2受体的分布
- Author:
Qiurong LI
;
Jian MA
;
Hao WANG
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
eicosapentaenoic acid;
lipid rafts;
IL-2 receptor
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine whether EPA exert effectively immunosuppressive function by altering distribution of IL-2R in membrane subdomains. Method: The human Jurkat E6-1 T cells were cultured in EPA-supplemented medium and the cells treated with stearic acid served as control. The effect of EPA on CD25 (IL-2? receptor) expression on the surface of T cells was investigated by flow cytometry. The lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2R?, IL-2R?, and IL-2R?c in fractions isolated and the effect of EPA treatment were determined by immunoblot and chemiluminescence. Results: EPA suppressed CD25 expression on the surface of T cells. IL-2R?, IL-2R?, and IL-2R?c were associated with lipid rafts of T cells, and these subunits were partly displaced from lipid rafts of EPA-treated T cells. Conclusion: Lipid rafts are functional subdomains for IL-2R signaling. EPA enrichment modifies distribution of IL-2R?, IL-2R?, and IL-2R?c in lipid rafts and EPA plays immunosuppressive roles probably by partly removing IL-2R from lipid rafts.
