EFFECT OF LITHIUM ON HIPPOCAMPUS NEURO NITRIC OXIDE SYNTHASE PROTEIN AND GENE EXPRESSION OF LEAD-EXPOSED RATS
- VernacularTitle:氯化锂对染铅大鼠海马nNOS蛋白与基因表达的影响
- Author:
Fang YANG
;
Jisheng LI
- Publication Type:Journal Article
- Keywords:
lithium;
lead;
hippocampus;
neuro nitric oxide synthase
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the antagonistic effect of lithium on the neurotoxicity of lead acetate and the possible mechanisms. Method: Wistar rats were randomly divided into control group, Pb group and four Pb+ LiCl groups fed with feed containing 3, 30, 300, 3 000 mg/kg LiCl respectively. All Pb exposed groups were given distilled water containing 0.2% PbAc. The changes of nNOS protein and gene expression in rats hippocampus were studied by ABC immunohistochemistry and the semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) method. Rusults: Compared with the control group, the number of nNOS positive neurons in CA1 area and the nNOS mRNA content of hippocampus of LiCl (3, 30 mg/kg) groups were increased,while those of Pb-exposed rats were significantly decreased. Compared with Pb groups, the number of nNOS positive neurons and the nNOS mRNA content of Pb+LiCl (3,30,300 mg/kg) groups were increased. In dentate gyrus, the changes of the number of nNOS positive neurons coincided with the changes in CA1 area, but those in CA3 area showed little difference. Conclusion: Low dose of lithium could resist the lead neurotoxcity obviously.
