Effects of cell adhesion molecule CD_(54) and CD_(44) on apoptosis inducted by momordin in erythroleukemic K562 Cells
- VernacularTitle:粘附分子CD_(54)、CD_(44)在苦瓜蛋白诱导K562细胞凋亡中的作用
- Author:
Shudao XIONG
;
Lihui YIN
;
Jingrong LI
;
Yixiang HAN
;
Shenghui ZHANG
;
Jianbo WU
;
Yue YAN
;
Guanwu LI
- Publication Type:Journal Article
- Keywords:
momordin;
K562;
apoptosis;
CD_(54);
CD_(44)
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the change of the expression of cell adhesion molecule CD_ 54 and CD_ 44 in erythroleukemic K562 apoptosis cells induced by momordin from momordica charantia seeds and study the effects of cell adhesion molecule CD_ 54 and CD_ 44 on cell apoptosis induced by momordin. METHODS: After the treatment of K562 cells with appropriated concentration momordin, CCK-8 test was employed to determine K562 cells growth; flow cytometry FACScan (FITC-Annexin V staining) and electron microscopy were used to detect apoptosis; The expression of CD_ 54 and CD_ 44 were examined by flow cytometry FACScan (FITC-CD_ 54 and PE-CD_ 44 staining). RESULTS: CCK-8 test showed K562 cells growth was significant inhibited by momordin; the apoptosis was detected by cell morphology and flow cytometry FACScan (FITC-Annexin V) in K562 cells after treatment by appropriated concentration momordin. The expressions of CD_ 54 and CD_ 44 in momordin treated K562 cells were 18.62 % and 1.32 % respectively, and in negative momordin treated K562 cells were 0.25 % and 0.17 % respectively, and momordin could up-expresses the protein of CD_ 54 18.37 % and CD_ 44 1.15 %. CONCLUSION: Momordin can markedly induce the K562 cell to apoptosis. The up-expressions of CD_ 54 exist in the process of apoptosis induced by momordin. The change of cell adhesion molecule maybe one of the key factors in the mechanisms of apoptosis induced by momordin, and its mechanism maybe involve in adhesion-dependent apoptosis.
